Table 4. Amplicon sequencing results and CT values for select clinical samples.
| n | Pathogenic rtPCR Positive, n (%) | UFI Assay Mean CT (Standard Deviation) | Amplicons Sequenced | Sequence Matches1 (n) | |
| Reference 16S rt PCR Positive 1 | 24 | 24 (100) | 28.33 (4.05) | 13 | L. interrogans, L. kirschneri, or L. noguchii (9) |
| L. borgpetersenii, L. santarosai, or L. weilii (3) | |||||
| L. meyeri, L. biflexa, or L. wobachii (1) | |||||
| flaB/lipL41 PCR and Reference 16S rt PCR Negative | 36 | 34 (94.4) | 32.62 (2.21) | 34 | L. interrogans, L. kirschneri, or L. noguchii (34) |
1
Given the highly conserved nature of this region, final species determinations cannot be made from amplicon sequences.