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. 2014 Nov 7;9(11):e112356. doi: 10.1371/journal.pone.0112356

Table 5. Amplicon sequencing and CT values for select clinical samples evaluated by MAT.

n Pathogenic rtPCR Positive, n (%) UFI Assay Mean CT (Standard Deviation) Amplicons Sequenced Sequence Matches1 (n)
MAT Positive 6 6 (100) 32.51 (2.27) 6 L. interrogans, L. kirschneri, or L. noguchii (6)
MAT Negative 49 47 (95.9) 30.49 (3.90) 39 L. interrogans, L. kirschneri, or L. noguchii (35)
L. borgpetersenii, L. santarosai, or L. weilii (3)
L. meyeri, L. biflexa, or L. wobachii (1)
1

Given the highly conserved nature of this region, final species determinations cannot be made from amplicon sequences.