Table 5. Amplicon sequencing and CT values for select clinical samples evaluated by MAT.
n | Pathogenic rtPCR Positive, n (%) | UFI Assay Mean CT (Standard Deviation) | Amplicons Sequenced | Sequence Matches1 (n) | |
MAT Positive | 6 | 6 (100) | 32.51 (2.27) | 6 | L. interrogans, L. kirschneri, or L. noguchii (6) |
MAT Negative | 49 | 47 (95.9) | 30.49 (3.90) | 39 | L. interrogans, L. kirschneri, or L. noguchii (35) |
L. borgpetersenii, L. santarosai, or L. weilii (3) | |||||
L. meyeri, L. biflexa, or L. wobachii (1) |
Given the highly conserved nature of this region, final species determinations cannot be made from amplicon sequences.