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. 2014 Nov 7;9(11):e112384. doi: 10.1371/journal.pone.0112384

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© 2014 Atta et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) RT-PCR gel of HGF mRNA expression. HGF mRNA expression was significantly (*, p<0.001) elevated in the saline-treated fibrotic (n = 14) compared to saline-treated healthy livers (n = 10) and further elevated (#, p<0.01) in the HGF-treated fibrotic (n = 13) compared with the saline-treated fibrotic and mMM-9-treated fibrotic (n = 10) livers (*, p<0.01). (C) Analysis of HGF protein concentration in the liver tissues. HGF protein concentration was significantly (*, p<0.001) elevated in the liver of the saline-treated fibrotic livers (n = 11) compared to saline-treated healthy livers (n = 10) and more elevated in the HGF-treated fibrotic (n = 14) compared to the saline-treated fibrotic (#, p<0.05) and mMMP-9-treated fibrotic (n = 10) livers (**, p<0.01). (D) Analysis of MMP-9 protein concentration in the liver tissues. MMP-9 protein concentration was significantly elevated in the mMMP-9-treated (n = 10) compared with healthy (n = 9), saline-treated fibrotic (n = 13) livers, HGF-treated (n = 12) livers (*, p<0.01) and HGF/mMMP-9-treated (n = 6) livers (#, p<0.05).