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. 2014 Nov 7;9(11):e112402. doi: 10.1371/journal.pone.0112402

Table 1. Primer Sequences for real-time PCR.

Gene Species Accession No. Primer Sequence Amplicon Length, nt
18S Rat X01117 5′-GTAACCCGTTGAACCCCATT-3′ 105
5′-CCATCCAATCGGTAGTAGCG-3′
IL-1β Sheep NM_001009465 5′-CGATGAGCTTCTGTGTGATG-3′ 120
5′-CTGTGAGAGGAGGTGGAGAG-3′
IL-6 Sheep NM_001009392 5′-CGCAAAGGTTATCATCATCC-3′ 107
5′-CCCAGGAACTACCACAATCA-3′
IL-8 Sheep NM_001009401 5′-CCTCAGTAAAGATGCCAATGA-3′ 82
5′-TGACAACCCTACACCAGACC-3′