Table 1. Primer Sequences for real-time PCR.
Gene | Species | Accession No. | Primer Sequence | Amplicon Length, nt |
18S | Rat | X01117 | 5′-GTAACCCGTTGAACCCCATT-3′ | 105 |
5′-CCATCCAATCGGTAGTAGCG-3′ | ||||
IL-1β | Sheep | NM_001009465 | 5′-CGATGAGCTTCTGTGTGATG-3′ | 120 |
5′-CTGTGAGAGGAGGTGGAGAG-3′ | ||||
IL-6 | Sheep | NM_001009392 | 5′-CGCAAAGGTTATCATCATCC-3′ | 107 |
5′-CCCAGGAACTACCACAATCA-3′ | ||||
IL-8 | Sheep | NM_001009401 | 5′-CCTCAGTAAAGATGCCAATGA-3′ | 82 |
5′-TGACAACCCTACACCAGACC-3′ |