Figure 8.

AST prevented H₂O₂-induced translocation of Bcl-2 family proteins in RLE-6TN cells. Cells were treated with 8 μM AST alone for 12 hrs and then co-treated with 65 μM H₂O₂ for 12 hrs. Then, cytosolic and mitochondrial fractions were prepared, and Bcl-2 member proteins were detected using specific antibodies by immunoblot. A representative blot from duplicate experiments is shown. #P < 0.01 versus cells treated with H₂O₂ alone.