Skip to main content
. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Aug 8;7(4):510–520. doi: 10.1007/s12195-014-0351-x

Figure 1.

Figure 1

Figure 1

Figure 1

Figure 1

Figure 1

Degradation of endogenous ATP attenuates shear stress-induced NO production. A-D. Sample traces of the NO response before (solid line) and after (dotted line) treatment with 1U/mL of apyrase. The steady-state concentration was offset to zero in order to show the individual NO response due to the step change (at 50s). Sample responses from a step change to A. 1 dyn/cm2 B. 6 dyn/cm2 C. 10 dyn/cm2. D. 20 dyn/cm2. E. Within an experiment, apyrase showed an inhibition of the shear stress-induced NO response; however, due to the variability between experiments, we normalized the responses for an untreated step change to 10 dyn/cm2 within each experiment. Comparisons of the Δ[NO] response before and after treatment with apyrase. Δ[NO] between untreated and apryase treated cells were found to be statistically significant for step changes to 6, 10 and 20 dyn/cm2 but not to 1 dyn/cm2. Δ[NO] for apyrase treated cells averaged 57% for 6 dyn/cm2, 77% for 10 dyn/cm2 and 80% for 20 dyn/cm2 of the untreated responses. (Mean and SEM, one-tailed t-test, *p<0.05, n=4)