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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Aug 8;7(4):510–520. doi: 10.1007/s12195-014-0351-x

Figure 10.

Figure 10

Inhibition of PKC attenuates ATP induced phosphorylation of eNOS. Prior to stimulation with 100 μM ATP with calcium, cells were treated with 30 μM of cheler for 2 min prior to stimulation. Cells were harvested before stimulation (t=0) and at time points 1, 3, 5, and 10 min after stimulation. All peNOS/eNOS ratios were normalized by t=0. Cheler treated cells showed an attenuated agonist stimulated phosphorylation of eNOS, which was statistically significant at 1 and 3 min (p<0.05 * one-tailed t-test, ATP w Ca+2 n=4, Cheler n=5).