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. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: Cell Mol Bioeng. 2014 Aug 8;7(4):510–520. doi: 10.1007/s12195-014-0351-x

Inhibition of purinergic receptors attenuates shear stress-induced NO production. Purinergic receptors were blocked with using the non-specific blocker suramin. Cells were incubated with 200 μM of suramin for 10 min prior to insertion into the chamber. Each membrane was exposed to a series of 4 step changes from 0.1 to 10 dyn/cm². A. Sample traces of the response for untreated (solid line) and suramin (dotted line) treated cells. The steady-state concentration was offset to zero in order to show the individual NO response due to the step change occurring at 50s. B. Bar graph comparing the Δ[NO] response to shear stress in untreated and suramin treated cells. The Δ[NO] response in cells treated with suramin averaged 40% of the untreated responses and were statistically significant. (Mean and SEM were plotted, one-tailed t-test. n=3 each, *p<0.05).

Inhibition of purinergic receptors attenuates shear stress-induced NO production. Purinergic receptors were blocked with using the non-specific blocker suramin. Cells were incubated with 200 μM of suramin for 10 min prior to insertion into the chamber. Each membrane was exposed to a series of 4 step changes from 0.1 to 10 dyn/cm². A. Sample traces of the response for untreated (solid line) and suramin (dotted line) treated cells. The steady-state concentration was offset to zero in order to show the individual NO response due to the step change occurring at 50s. B. Bar graph comparing the Δ[NO] response to shear stress in untreated and suramin treated cells. The Δ[NO] response in cells treated with suramin averaged 40% of the untreated responses and were statistically significant. (Mean and SEM were plotted, one-tailed t-test. n=3 each, *p<0.05).