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ATP induced eNOS phosphorylation is dependent on increases in intracellular calcium. Cells were simulated with 100 μM ATP with calcium. Prior to stimulation, cells were treated with 30 μM of BAPTA for 30 min. Cells were harvested before stimulation (t=0) and at time points 1, 3, 5, and 10 min after stimulation. All peNOS/eNOS ratios were normalized by t=0. Treatment with BAPTA abolished the eNOS phosphorylation response. (p<0.05 *; p<0.01 ** one-tailed t-test, n=4 each)
