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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Cell Mol Bioeng. 2014 Aug 8;7(4):510–520. doi: 10.1007/s12195-014-0351-x

Figure 8.

Figure 8

ATP induced eNOS phosphorylation is dependent on CCE. Cells were simulated with 100 μM ATP with calcium. Prior to stimulation, cells were treated with 50 μM of SKF-96365 for 10 min. Cells were harvested before stimulation (t=0) and at time points 1, 3, 5, and 10 min after stimulation. All peNOS/eNOS ratios were normalized by t=0. SKF-96365 treated cells (SKF) show an attenuated eNOS phosphorylation at 1 and 3 min, which was statistically significant. (p<0.05 * one-tailed t-test, ATP w Ca+2 n=4, SKF n=5)