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. Author manuscript; available in PMC: 2015 Dec 1.

Published in final edited form as: Cell Mol Bioeng. 2014 Aug 8;7(4):510–520. doi: 10.1007/s12195-014-0351-x

Inhibition of PKC attenuates the ATP stimulated sustained calcium response. Prior to stimulation with 500 μL of 100 μM ATP with calcium, cells were incubated with 30 μM of the general PKC inhibitor cheler for 2 min. A. Representative traces of the calcium response in untreated (solid line) and cheler (dotted line) treated cells to 500 μL of 100 μM ATP with calcium. B. Bar graph representing average responses for each condition at the peak response, 60(s) and 100(s). Cheler treated cells showed attenuation of the peak and sustained calcium response. Average responses represent multiple coverslips (cs) and total cell count among coverslips (n) Untreated: #cs= 3, n=120, Cheler: #cs=4, n=160, two-tailed t-test ***p<0.0001.

Inhibition of PKC attenuates the ATP stimulated sustained calcium response. Prior to stimulation with 500 μL of 100 μM ATP with calcium, cells were incubated with 30 μM of the general PKC inhibitor cheler for 2 min. A. Representative traces of the calcium response in untreated (solid line) and cheler (dotted line) treated cells to 500 μL of 100 μM ATP with calcium. B. Bar graph representing average responses for each condition at the peak response, 60(s) and 100(s). Cheler treated cells showed attenuation of the peak and sustained calcium response. Average responses represent multiple coverslips (cs) and total cell count among coverslips (n) Untreated: #cs= 3, n=120, Cheler: #cs=4, n=160, two-tailed t-test ***p<0.0001.