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. Author manuscript; available in PMC: 2015 May 6.
Published in final edited form as: Nature. 2014 Sep 3;515(7525):138–142. doi: 10.1038/nature13601

Figure 4. Binding or neutralization in the context of a lipid membrane.

Figure 4

a, Staining of cell surface expressed HIVJRFL Env. b, ELISA assay of antibody binding to WT or SOS HIVJRFL VLPs. The CD4-inducible 39F antibody or gp41-specific 7B2 are used as controls. c, Access to the HIVJRFL Env trimer on pseudovirions based upon washing the antibody-pseudovirion mixture prior to infecting cells. d, Kinetic assay of HIVJRFL neutralization. See Methods for a description of individual formats. e, Schematic of conformational change resulting in raising of the trimer spike required to permit access of 35O22 to its epitope.