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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Am Soc Mass Spectrom. 2014 Sep 16;25(12):2093–2102. doi: 10.1007/s13361-014-0973-1

Figure 4.

Figure 4

HDX MS contact areas: the interacting region of exEGFR (grey with color-coded residues) with Adnectin 1 (green) (PBD ID: 3QWQ Ref. [19]). The backbone amide hydrogens are illustrated as blue balls. The peptides that were found to have significant protection from deuteration upon Adnectin 1 binding are shown in pink (1-19, 96-108) and the ones with moderate protection from deuteration are shown in yellow (45-54). Targeted ETD reveals that exEGFR residues cyan (K13), red (L14), orange (T15), blue (Q16), magenta (L17) and bronze (G18) were protected from deuteration when bound to Adnectin 1, overlapping with the same residues previously confirmed by X-ray crystal structure (T15, Q16, L14, and G18) [19]. The view of the main figure is from a spot indicated with a small black arrow in the lower left inset.