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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Nature. 2014 Jul 13;513(7516):100–104. doi: 10.1038/nature13528

Figure 2. LLC cell-conditioned medium stimulates thermogenic gene expression in fat cells.

Figure 2

a-c,ParentalLLC cells or the subclones were cultured in serum-free medium for 24 hr. Primary adipocytes (n = 3 in a and b) were treated for 24 hr with the LLC cell-conditioned media (a and c) orthe filtered fractions (b). d, Gene expression heat map of the secreted factors identified in the microarray analysis. e, Primary adipocytes were treated with the indicated proteins (1 μg/ml) for 24 hr (n = 3). mRNA levels were determined by RT-qPCR. Ap2 and Pparg are controls for adipocyte differentiation.Values are means ± SEM. Statistics by two-tailed t-test. *P < 0.05, **P < 0.005, ***P < 0.0005 compared with the control group.