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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Nature. 2014 Jul 13;513(7516):100–104. doi: 10.1038/nature13528

Figure 4. Neutralization of PTHrP prevents tumor-induced adipose tissue browning.

Figure 4

a, Plasma PTHrP levels in mice bearing LLC tumors up to 21 days (n = 6). b-g, Mice inoculated with LLC cells received IgG or anti-PTHrP (10 mg/kg body weight) every 3 days from day 6 to day 15 and were sacrificed at day 16 (n = 4, 5 and 6 for the NTB, IgG and anti-PTHrP groups respectively). Carcass weight (b), weight of tumors (c), fat and muscle tissues (d) and H&E staining of adipose tissues were shown (e). mRNA levels in eWAT (f) and iBAT (g) were measured by RT-qPCR. h, Mice were treated similarly to b-g except that they were placed into metabolic cages at day 4 to measure O2 consumption (n = 6 for the NTB group and 5 for the other groups). Values are means ± SEM. Statistics by two-tailed t-test. (*) compares the NTB and IgG groups. (#) refers to differences between the IgG and anti-PTHrP groups. *P < 0.05, **P < 0.005, ***P < 0.0005, #P < 0.05, ##P < 0.005, ###P < 0.0005.