Extended Data Figure 2. Fat-specific Prdm16-deficient mice are resistant to LLC tumor-induced adipose tissue browning and wasting.
Wild-type (WT) and Prdm16-knockout (KO) mice inoculated with LLC cells were sacrificed at day 11 (n = 5 for the KO-LLC group and 6 for the other groups). Tissue weight (a) and tumor weight (b) were measured. Gene expression changes in iWAT (c), eWAT (d), iBAT (e) and gastrocnemius muscle (f) were determined by RT-qPCR. Values are means ± SEM. Statistics by two-tailed t-test. (*) refers to differences between the NTB and LLC groups. (#) refers to differences between the WT and KO groups. *P < 0.05, **P < 0.005, ***P < 0.0005, #P < 0.05, ##P < 0.005, ###P < 0.0005.