Fig.5.

Effect of RNase L on splenic immune cell subtypes and T-cell function A. Lymphocytes isolated from RNase L^+/+ and ^−/− mice (n=4/group) were incubated with retinal endothelial cells at 1:5 at 37°C for 24 hr. Cell viability was measured by trypan blue exclusion. Samples were triplicates and the data are present as Mean ± SD, ***p<0.048. B and C. Treg cells in the spleens of RNase L^+/+ (WT) and ^−/− (KO) mice were analyzed for the expression of CD4 and CD25 by flow cytometric analysis. The percentages of CD4⁺CD25⁺Treg cells and CD4⁺CD25⁺Foxp3 Terg cells in total CD4⁺T cells are present as Mean ± SD (n= 4). *P< 0.05; **p<0.025. D and E. Six-week-old male RNase L^+/+ and ^−/− mice (3mice/group) were treated with or without 25 μg LPS for each mouse every other day for a week. The splenic cells were subjected to cell sorting after labeled with CD4, CD8, B220, and IgD alone or combined.