Figure 6.

The inner retina of EE and ST mice. Antibody staining of retinal cell types in rd10 mice, kept in EE (A, C and E) and in ST conditions (B, D, and F), respectively. A and B: Znp1 staining of cone bipolar cells (green signal) reveals extensive loss of dendrites in the outer plexiform layer (OPL) of the samples in A and B and better preservation of axonal arbors in the inner plexiform layer (IPL) of the enriched environment (EE) retinas (A). Horizontal cells (red signal) stained with calbindin D antibodies appear devoid of dendrites in both cases. C and D: Cholinergic amacrines, stained with ChAT antibodies (red signal), are well preserved in the EE (C) and standard laboratory (ST) (D) samples, and the two parallel, cholinergic bands run in the IPL at the expected heights. Similarly, ganglion cells, stained green by neurofilament antibodies, are well preserved, with axons running in bundles (arrows). E and F: Calretinin staining of amacrine cells (red signal) reveals the three dendritic bands regularly laminating the IPL, in the EE (E) and ST (F) retinas. Neurofilament labeling (green signal) shows fine ganglion cell dendrites running in the IPL and fiber clusters (arrows) at the inner retinal aspect. In the outer retina, residual processes belonging to axonal arborizations of horizontal cells are lightly stained in both specimens.