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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Cell Signal. 2014 Jul 13;26(11):2406–2411. doi: 10.1016/j.cellsig.2014.07.005

Figure 5. LPA1I325A, LPA1Y85A, and LPA1L87A mutants accumulate within the ER.

Figure 5

A. LPA1-V5 over-expressed cell lysates were treated with or without PNGase F, followed by V5 and β-actin immunobloting. B. LPA1-V5 was co-expressed with Sar1aH79G-Flag or Sar1aWt- Flag in MLE12 cells. Cell lysates were analyzed for V5, Flag, and β-actin immunobloting. C. LPA1I325A-V5, LPA1Y85A-V5, or LPA1L87A-V5 was co-overexpressed with Sar1aWt-Flag in MLE12 cells. Cell lysates were analyzed for V5 and Flag immunobloting. D. LPA1 wild type and point mutants with V5 tag were co-overexpressed with DsRed2-ER in MLE12 cells. Immunostaining were performed with a V5 antibody (green). DsRed2-ER (ER-RED) shows in red and nuclei were stained with DAPI (blue).