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. Author manuscript; available in PMC: 2014 Nov 10.
Published in final edited form as: Gut. 2012 Jun 8;62(5):695–707. doi: 10.1136/gutjnl-2011-301775

Figure 5. WFA inhibits NOD2-dependent NF-κB signaling.

Figure 5

(a) SW480 cells were transfected with NF-κB-luciferase wild-type (NF-κB-luc-WT) or mutant with NF-κB binding sites deleted (NF-κB-luc-Mut) (200ng) and the internal control renilla (100ng). After 16-18h cells were treated for 6h with L18-MDP (1μg/ml) or for 2h with TNF-α (50ng/ml) and harvested for luciferase assays. The data are derived from triplicate readings.

(b) SW480 cells were transfected with NF-κB-luciferase wild-type (NF-κB-luc-WT) or mutant with NF-κB binding sites deleted (NF-κB-luc-Mut) (200ng) and the internal control renilla (100ng). After 16-18h cells were treated for 6h with L18-MDP (1μg/ml) or for 2h with TNF-α (50ng/ml) in the presence of WFA (2h) or DMSO control (2h) and harvested for luciferase assays. The data are derived from triplicate readings. * = P<0.05, ns = not significant.