Figure 2.
Unitary Production of Excitatory Neurons by RGPs
(A) 3D reconstruction images of representative symmetric proliferative (left) and asymmetric neurogenic (right) clones. Schematics of the clone are shown at the top. RG, radial glia; N, neuron; IP, intermediate progenitor.
(B) Percentage of symmetric proliferative division versus asymmetric neurogenic division at different embryonic stages.
(C) Quantification of the size of asymmetric neurogenic clones labeled at E10–E12 (n = 109).
(D) Clone size distribution of the asymmetric neurogenic clones at E10–E12 fitted by a Gaussian distribution, indicating an average RGP output of ∼8–9 neurons (mean μ0 = 8.4, SD δ = 2.6; fitting error = 5.3%; blue broken line; termed “Unitary Gaussian”).
(E) Gaussian fitting of the overall clone size variation. The 192 clones with a size of up to 50 neurons were fitted by the sum (black line) of a series of Gaussians centered on integer multiples of the mean of Unitary Gaussian in D (1μ0, 2μ0, 3μ0; colored lines; higher-order Gaussians are not plotted for clarity).
(F) Quantification of the size of asymmetric neurogenic clones located in different neocortical areas (SS, 7.9 ± 0.3, n = 44; MO, 8.1 ± 0.7, n = 10; AUD, 7.3 ± 0.6, n = 15; VISal, 9.0 ± 1.0, n = 2; PTLp, 8.8 ± 0.7, n = 5; Medial, 7.6 ± 1.2, n = 10). SS, somatosensory cortex; MO, motor cortex; AUD, auditory cortex; VISal, visual cortex; PTLp, posterior parietal association areas; Medial, including anterior cingulate area, dorsal peduncular area, infralimbic area, prelimbic area, and retrosplenial area.
Data are presented as mean ± SEM. n.s., not significant. See also Figures S3 and S4.