Figure 4.

Individual RGPs Produce Both Deep- and Superficial-Layer Excitatory Neurons

(A) 3D reconstruction images of representative clones labeled at different embryonic stages. Note that all clones contain both superficial (2–4) and deep (5–6) layer neurons.

(B) Confocal images of an E10 clone stained with the antibodies against EGFP (green), tdTomato (red), BRN2 (white), and CTIP2 (cyan) and with DAPI (blue). High-magnification images of representative superficial (B’) and deep (B’’) layer neurons (broken lines) are shown at the bottom. Arrows indicate neurons positive for BRN2, arrowheads indicate neurons positive for CTIP2, and open arrowheads indicate neurons negative for BRN2 or CTIP2. Scale bars, 100 μm and 25 μm.

(C) Percentage of clones containing both superficial and deep-layer neurons versus those containing only superficial- or deep-layer neurons.

(D) Percentage of neurons in the clones located in superficial or deep layers.

See also Figure S5.