Figure S3.
No Substantial Apoptosis in the Embryonic and Postnatal Neocortex, Related to Figure 2
(A) 3-D reconstruction image of a single colored (green fluorescent only) G2-X clone.
(B) Quantification of apoptosis rate of the daughter cell of dividing progenitors inferred from the occurrence of single colored G2-X clones. Data are presented as mean ± SEM. Note that the rate of the daughter cell apoptosis is less than 4%–7%.
(C) Confocal images of E12, E14, E16 and E18 neocortices stained with the antibody against Cleaved Caspase-3 (green), a marker for apoptotic cells, and with DAPI (blue). Note no substantial apoptosis in the embryonic neocortex. Scale bar: 100 μm.
(D) Quantification of the size of clones labeled at E12 and analyzed at E18, P7-10 and P21-30. Data are presented as mean ± SEM. n.s., not significant. Note that the clonal size is similar at different postnatal stages, indicating no substantial excitatory neuron apoptosis postnatally.
(E) Confocal images of P3, P5 and P7 neocortices stained with the antibody against Cleaved Caspase-3 (green) and with DAPI (blue). Scale bar: 100 μm.