Figure S5.

G₂-X Clones Contain Both Superficial- and Deep-Layer Neurons, Related to Figure 4

(A) Confocal images of a representative asymmetric neurogenic G₂-X clone stained with the antibodies against EGFP (green), tdTomato (red), BRN2 (white) and CTIP2 (cyan), and with DAPI (blue). High magnification image of representative superficial (A’) and deep (A’’) layer neurons (broken lines) are shown to the right. Arrows indicate neurons positive for BRN2, arrowheads indicate neurons positive for CTIP2, and open arrowheads indicate neurons negative for BRN2 or CTIP2. Scale bars: 100 μm and 20 μm.

(B) Confocal images of a representative symmetric proliferative G₂-X clone stained with the antibodies against EGFP (green), tdTomato (red), CUX1 (white) and CTIP2 (cyan), and with DAPI (blue). Arrows indicate neurons positive for CUX1, arrowheads indicate neurons positive for CTIP2, and open arrowheads indicate neurons negative for CUX1 or CTIP2. High magnification images of representative superficial (B’ and B’’) and deep (B’’’, broken lines) layer neurons are shown to the right. Scale bars: 100 μm and 20 μm.

(C) Percentage of L2-4 and L5-6 neurons of individual clones labeled at E10-12 that are positive for BRN2+ or CUX1+ and CTIP2+, respectively. Note all the clones contain BRN2+ or CUX1+ and CTIP2+ neurons.

(D) Percentage of clones labeled at E10-12 that contain both BRN2+ or CUX1+ and CTIP2+ neurons.