Figure 5.

Pharmacokinetics and receptor occupancy ASKP1240 were administered intravenously once on day 0. The serum ASKP1240 level was measured by an ELISA. To evaluate the receptor occupancy, the peripheral CD20+ cells in the collected blood samples were stained with biotinylated ASKP1240 plus PE-labeled streptavidin. The serum ASKP1240 concentration and the geoMFI of PE in the CD20+ lymphocytes are expressed as the means ± SE of three individuals (A, B). The correlation between the serum ASKP1240 concentration and receptor occupancy was plotted (C). ELISA, enzyme-linked immunosorbent assay; geoMFI, geometric mean fluorescence intensity; PE, phycoerythrin.