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. 2013 Jun 28;12(5):742–751. doi: 10.1111/acel.12101

Figure 2.

Figure 2

Translational control of atf-5 gene expression. (A) Schema of uORFs within the atf-5 5′-UTR. The amino acid length of the predicted translated uORF and the coding sequence of atf-5 gene are shown. (B) Confocal and bright field images of 1-day-old transgenic worms expressing the translational fusion of the intact atf-5 (left box) or the uORF-less atf-5 transgene (right box) under normal feeding conditions. A larger magnification of the area in the red box is shown on the top left. White arrows indicate fluorescent nuclei, and white arrowheads show regions of autofluorescence. All images were taken at 20× magnification under the same microscopy settings (scale bar: 50 μm). The levels of atf-5 mRNA in N2, BRF140, and BRF152 worms, normalized to ama-1(mRNA), were quantified using qRT–PCR.