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Loss of GCN-2 function affects lifespan only under nutrient stress. Survival curves of (A) N2, gcn-2, and atf-5 mutants fed OP50 bacteria (B) N2 worms treated with gcn-2(RNAi) or atf-5(RNAi) from L4s (C) eat-2(ad465) and eat-2(ad465);gcn-2(ok871) fed OP50 bacteria (D) N2 and gcn-2(ok871) treated with let-363(RNAi) from their first day of adulthood (E) N2 and gcn-2(ok871) treated with rsks-1(RNAi) from L4s (F) N2 and ife-2(ok306) treated with gcn-2(RNAi) from L4s.
