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. 2013 Jun 28;12(5):742–751. doi: 10.1111/acel.12101

Figure 5.

Figure 5

General control nonderepressible-2 influences the induction of pha-4 and its downstream targets in response to TOR inactivation. qRT–PCR of pha-4(mRNA) in (A) 1-day adults of N2 and gcn-2(ok871) treated with let-363(RNAi) from L3 stage; N2, eat-2, and eat-2;gcn-2 young adults raised on OP-50 bacteria at 20 °C; and in the F1 progenies (L2–L3 stage) of N2 and gcn-2(ok871) treated with rrt-1(RNAi) (B) qRT–PCR of sod-1, sod-2, sod-3, sod-4, mtl-1, and lgg-1 on 1-day adults of N2 and gcn-2(ok871) treated with let-363(RNAi) from L3 stage. Quantification of each mRNA level, relative to ama-1 mRNA and the mean ± SD of biological triplicates are shown. The asterisks represent statistical significant difference from N2/Control or gcn-2/Control (*P < 0.05, ** P < 0.01, ***P < 0.001 in unpaired t-test).