Table 2.
Lifespan experiments in RNAi plates*
| Strain/RNAi | Treatment/ IPTG† | Median/Max lifespan (days)‡ | Mean lifespan ± SEM (days)§ | Number (T/C)¶ | P-value against control** | P-value against specific control†† | |
|---|---|---|---|---|---|---|---|
| Fig. 4B | N2/Control | 20 °C/1 mm | 21/30 | 20.83 ± 0.44 | 83/3 | ||
| N2/gcn-2(RNAi) | ≫ | 21/31.3 | 20.67 ± 0.33 | 88/3 | 0.6489 | ||
| N2/atf-5(RNAi) | ≫ | 20/29 | 19.5 ± 0.29 | 70/5 | 0.5680 | ||
| Fig. S4 A | N2/Control | 20 °C/1 mm | 21/30.1 | 21 ± 0.58 | 124/5 | ||
| N2/rrt-1(RNAi) | ≫ | 10/16.1 | 10 ± 0.58 | 119/10 | <0.0001 | ||
| N2/krs-1(RNAi) | ≫ | 8/11.5 | 7.83 ± 0.16 | 124/6 | <0.0001 | ||
| Fig. S4 A | N2/Control | 20 °C/1 mm | 22/30.4 | 22 ± 1 | 118/9 | ||
| N2/rrt-1(RNAi) | ≫ | 13/20.6 | 13 ± 0.57 | 125/1 | <0.0001 | ||
| gcn-2(ok871)/Control | ≫ | 21/30.4 | 22.17 ± 1.69 | 117/5 | 0.0978 | ||
| gcn-2(ok871)/rrt-1(RNAi) | ≫ | 13/19.1 | 12.67 ± 0.67 | 108/6 | <0.0001 | 0.4078 | |
| Fig. S4 B | N2/Control | 20 °C/0.25 mm | 22/30.6 | 22.50 ± 0.29 | 80/5 | ||
| N2/rrt-1(RNAi) | ≫ | 19/28.3 | 18.83 ± 0.44 | 140/5 | <0.0001 | ||
| gcn-2(ok871)/Control | ≫ | 22/30.7 | 21.67 ± 0.67 | 76/8 | 0.6962 | ||
| gcn-2(ok871)/rrt-1(RNAi) | ≫ | 16/27.2 | 16.33 ± 0.33 | 138/2 | <0.0001 | <0.0001 | |
| Fig. S4 B | N2/Control | 20 °C/0.25 mm | 20/28.6 | 19.67 ± 0.67 | 91/5 | ||
| N2/rrt-1(RNAi) | ≫ | 17/25.8 | 16.33 ± 0.33 | 185/8 | <0.0001 | ||
| gcn-2(ok880)/Control | ≫ | 19/26 | 18.67 ± 0.33 | 92/2 | 0.1081 | ||
| gcn-2(ok880)/rrt-1(RNAi) | ≫ | 15/22.9 | 14.83 ± 0.16 | 177/6 | <0.0001 | <0.0001 | |
| Fig. S4 B | N2/Control | 20 °C/0.25 mm | 21/28.6 | 21.33 ± 0.67 | 82/12 | ||
| N2/krs-1(RNAi) | ≫ | 12/14.4 | 12 ± 0.0 | 96/9 | <0.0001 | ||
| gcn-2(ok871)/Control | ≫ | 22/27.9 | 22 ± 1 | 83/13 | 0.9383 | ||
| gcn-2(ok871)/krs-1(RNAi) | ≫ | 11/12.3 | 11 ± 0.0 | 79/9 | <0.0001 | <0.0001 | |
| Fig. 4D | N2/Control | 20 °C/0.25 mm | 22/31.7 | 21.5 ± 0.5 | 66/2 | ||
| N2/let-363(RNAi) | ≫ | 30/39 | 29.63 ± 1.14 | 120/3 | <0.0001 | ||
| gcn-2(ok871)/Control | ≫ | 21/31.2 | 21.25 ± 0.75 | 69/2 | 0.2995 | ||
| gcn-2(ok871)/let-363(RNAi) | ≫ | 24/34.4 | 24 ± 1.08 | 113/4 | 0.0279 | ||
| atf-5(ok576)/Control | ≫ | 21/30.4 | 21.5 ± 0.5 | 97/1 | 0.0456 | ||
| atf-5(ok576)/let-363(RNAi) | ≫ | 27/35.3 | 27.13 ± 0.77 | 111/3 | <0.0001 | ||
| Fig. 4F | N2/Control | 20 °C/1 mm | 21/29.2 | 21 ± 0.58 | 119/2 | ||
| ife-2(ok306)/Control | ≫ | 23/32.4 | 23 ± 0.41 | 133/7 | 0.0002 | ||
| ife-2(ok306)/gcn-2(RNAi) | ≫ | 23/31.5 | 23.25 ± 0.25 | 124/5 | 0.1072 | ||
| ife-2(ok306)/atf-5(RNAi) | ≫ | 23/30.3 | 22.83 ± 0.44 | 123/4 | 0.1696 | ||
| Fig. 4E | N2/Control | 20 °C/1 mm | 20/30 | 19.75 ± 0.48 | 112/12 | ||
| N2/rsks-1(RNAi) | ≫ | 23/34.1 | 23.25 ± 0.75 | 135/5 | <0.0001 | ||
| gcn-2(ok871)/Control | ≫ | 22/30.8 | 21.88 ± 0.87 | 131/18 | 0.1485 | ||
| gcn-2(ok871)/rsks-1(RNAi) | ≫ | 22/30.2 | 22.13 ± 0.72 | 130/8 | 0.6481 | ||
| atf-5(ok576)/Control | ≫ | 19/28 | 19 ± 0.41 | 101/15 | 0.0495 | ||
| atf-5(ok576)/rsks-1(RNAi) | ≫ | 20/30.7 | 20.75 ± 0.75 | 134/18 | 0.0162 |
IPTG, isopropylb-D-thiogalactopyranoside.
*
Data from representative experiments are shown. Data from independent repeats of each longevity assay are shown in Table S3 (Supporting information).
†
IPTG final concentration in bacterial cultures.
‡
Max lifespan is the mean of the last 10% surviving worms.
§
Mean lifespan and standard error of the mean (SEM) of 2–4 plates.
¶
Total number (T) of dead and censored (C) worms/censored (C).
**
P-value from log-rank test comparing a RNAi-treated strain to isogenic Control strain. P < 0.05 is considered statistically significant.
††
P-value from log-rank test comparing a mutant strain to the equal treated N2 population, for example gcn-2/Control vs. N2/Control or gcn-2/RNAi vs. N2/RNAi.