Figure 4.

The protein expression of AMFR and NOTCH1 was upregulated in CRC and negatively correlated with miR-139-5p expression. (A and B) Immunohistochemical staining of AMFR and NOTCH1 in CRC tissues and corresponding noncancerous tissues (NCTs). Brown cytoplasmic AMFR/NOTCH1 staining was strong in CRC tissues but nearly absent in the normal epithelia. AMFR and NOTCH1 protein expression were frequently increased in the tumor tissues compared with the matched NCTs. (C) The expression levels of AMFR and NOTCH1 negatively correlated with the miR-139-5p levels in the CRC tissues. (D) Survival analysis based on the expression levels of NOTCH1 protein. The groups were ranked according to the NOTCH1 staining intensity