Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1972 Sep;6(3):414–415. doi: 10.1128/iai.6.3.414-415.1972

Purification of Polysaccharide Antigen from Leptospira biflexa Strain Urawa

Tadayoshi Taniyama 1,2, Yasutake Yanagihara 1,2, Ichiji Mifuchi 1,2, Ichiro Azuma 1,2,1, Yuichi Yamamura 1,2
PMCID: PMC422549  PMID: 4637612

Abstract

Serologically active polysaccharide was isolated from the cells of Leptospira biflexa strain Urawa and purified. The constituents of this polysaccharide were characterized, and its serological specificity was partially examined.

Full text

PDF
415

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMINOFF D., MORGAN W. T. J., WATKINS W. M. The isolation and properties of the human blood-group a substance. Biochem J. 1950 Apr;46(4):426–438. doi: 10.1042/bj0460426. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baseman J. B., Cox C. D. Intermediate energy metabolism of Leptospira. J Bacteriol. 1969 Mar;97(3):992–1000. doi: 10.1128/jb.97.3.992-1000.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CHRISTIANSEN A. H. Studies on the antigenic structure of Treponema pallidum. 2. Isolation and purification of polysaccharides from Reiter's apathogenic strain. Acta Pathol Microbiol Scand. 1962;56:166–176. [PubMed] [Google Scholar]
  4. CHRISTIANSEN A. H. Studies on the antigenic structure of Treponema pallidum. 3. Antigenic properties of polysaccharides isolated form Reiter's apathogenic strain. Acta Pathol Microbiol Scand. 1962;56:177–187. doi: 10.1111/j.1699-0463.1962.tb04179.x. [DOI] [PubMed] [Google Scholar]
  5. ELIAN M., NICOARA I. THE USE OF LEPTOSPIRA BIFLEXA PATOC ANTIGEN IN FIELD INVESTIGATIONS OF LEPTOSPIROSIS. Bull World Health Organ. 1964;31:359–363. [PMC free article] [PubMed] [Google Scholar]
  6. Elson L. A., Morgan W. T. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27(6):1824–1828. doi: 10.1042/bj0271824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Nell E. E., Hardy P. H. Studies on the chemical composition and immunologic properties of a polysaccharide from the Reiter treponeme. Immunochemistry. 1966 May;3(3):233–245. doi: 10.1016/0019-2791(66)90187-x. [DOI] [PubMed] [Google Scholar]
  9. OUCHTERLONY O. Antigen-antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta Pathol Microbiol Scand. 1953;32(2):230–240. [PubMed] [Google Scholar]
  10. PILLOT J., DUPOUEY P. COMPOSITION ANTIG'ENIQUE DES TR'EPON'EMES V. ETUDE IMMUNOLOGIQUE DES ANTIG'ENES POLYOSIDIQUES DU TR'EPON'EME REITER. DISCUSSION. Ann Inst Pasteur (Paris) 1964 Apr;106:617–624. [PubMed] [Google Scholar]
  11. PILLOT J., DUPOUEY P. COMPOSITION ANTIG'ENIQUE DES TR'EPON'EMES. IV. SOLUBILISATION ET PURIFICATION DES ANTIG'ENES POLYOSIDIQUES DU TR'EPON'EME REITER. Ann Inst Pasteur (Paris) 1964 Mar;106:456–468. [PubMed] [Google Scholar]
  12. SCHNEIDER M. D. Isolation and chemical composition of complement-fixing antigens from leptospires. Proc Soc Exp Biol Med. 1954 Jan;85(1):32–37. doi: 10.3181/00379727-85-20776. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES