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Map of plasmid pFS7.Ps2: constitutive promoter; xylS: gene encoding Pm activator; luc: gene encoding luciferase; Pm: positively regulated promoter; bla: ampicillin resistance gene encoding β-lactamase; t1: rrnBT1T2 bidirectional transcriptional terminator; trfA: gene encoding the replication protein; oriV: origin of vegetative replication; kan: kanamycin resistance gene; oriT: origin of conjugal transfer. The DNA sequence of the overlapping stop-start codon is depicted.
