Table 1.
Average release of the proinflammatory cytokines IL-1α, IL-8, TNFα (pg/ml) and LDH (measured by optical absorbance at 490 nm) from RHE 24 hours after topical treatment with S. epidermidis , P. acnes and S. aureus at different concentrations
| Untreated control | 1×10 7 CFU | 1×10 8 CFU | 1×10 9 CFU | |
|---|---|---|---|---|
|
S. epidermidis
|
|
|||
| [IL-1α] released |
100 ± 3.5 |
116 ± 111.2 |
143.6 ± 156.8 |
186.8 ± 215.8 |
| [IL-8] released |
100 ± 18.4 |
151.7 ± 248.8 |
118.9 ± 156 |
193.4 ± 239.5* |
| [TNFα] released |
100 ± 0.6 |
124.4 ± 35.6 |
138 ± 77.1 |
151.8 ± 80.7 |
| LDH absorbance |
100 ± 0.03 |
83.6 ± 63.2 |
82.6 ± 62.9 |
88.6 ± 58.2 |
|
P. acnes
|
|
|||
| [IL-1α] released |
100 ± 8.28 |
123.1 ± 100.3 |
122 ± 91 |
133.4 ± 112.1 |
| [IL-8] released |
100 ± 42.08 |
120.7 ± 129.6 |
109.5 ± 97.3 |
107.1 ± 84.9 |
| [TNFα] released |
100 ± 1.6 |
162.6 ± 158.1 |
115.7 ± 126.1 |
88.6 ± 113.6 |
| LDH absorbance |
100 ± 0.04 |
100.2 ± 148.4 |
108.8 ± 161.6 |
102.1 ± 142.8 |
|
S. aureus
|
|
|||
| [IL-1α] released |
100 ± 2.3 |
157.6 ± 303.8 |
1062.1 ± 3556.2 |
1251.7 ± 3735.6 |
| [IL-8] released |
100 ± 12.1 |
60.4 ± 27.7 |
93.1 ± 95.1 |
73 ± 2.6 |
| LDH absorbance | 100 ± 0.1 | 273.3 ± 357.5 | 505.2 ± 1002 | 666.4 ± 1321 |
Results are expressed as percentage of the untreated control.
*Single asterisk indicate statistically significant differences (P < 0.05) versus the untreated control.