Table 1.
Chemico-physical parameters of the recombinant proteins as produced upon cloning in pET32b or pColdI
| Protein | Length (aa) | Molecular mass (Da) | Theoretical pI |
|---|---|---|---|
| Trx-TB10.4 |
261 |
28,133.6 |
5.06 |
| TB10.4 |
103 |
11,076.3 |
4.44 |
| Trx-Ag85B |
450 |
48,405.2 |
5.11 |
| Ag85B |
292 |
31,347.9 |
4.77 |
| Trx-full |
546 |
58,777.7 |
4.99 |
| His-full1 |
414 |
44,865.9 |
5.11 |
|
full1 |
388 |
41,720.5 |
4.71 |
| His-full2 | 397 | 43,060.0 | 5.53 |
Theoretical isoelectric point (pI) was calculated by ExPASyBioinformatic Resource Portal (http://www.expasy.org/) using ProtParam tool.