Table 2.
Production yield of purified recombinant Trx-TB10.4, Trx-Ag85B (chimeric and single antigens, after proteolytic cleavage), and His- full 2 by E. coli cells grown at flask and bioreactor scales
| Protein | Growth conditions | Biomass at the harvest time a (g/L) | Pure chimeric protein b (mg/L) | Pure mature protein (mg/L) |
|---|---|---|---|---|
| Trx-TB10.4 |
Flasks |
4.8 |
12 |
4c |
| |
Bioreactor |
8.5 |
80 |
25c |
| Trx-Ag85B |
Flasks |
10.5 |
9 |
4.7c |
| |
Bioreactor |
19.5 |
30 |
16c |
| His-full2 |
Flasks |
6.5 |
/ |
0.5 |
| Bioreactor | 6.5 | / | 2.5 |
agrams of E. coli cells (wet weight) per liter of cultivation broth at the harvest time.
bmilligrams of protein obtained upon purification by nickel-affinity chromatography per liter of cultivation broth.
cmilligrams of protein obtained upon proteolytic cleavage and separation by nickel-affinity chromatography per liter of cultivation broth.