Table 1.

Yields and composition of cell wall material of fruitlet, expanding, mature and ripe ‘Royal Gala’ (RG) and ‘Scifresh’ (SF) apples

Stage	Fruitlet		Expanding		Mature		Ripe
	40 DAFB		70 DAFB		120/140 DAFB		20 weeks
Cultivar	RG	SF	RG	SF	RG	SF	RG	SF
Yield cell wall material	64.3 ± 5.3	51.2 ± 8.3	19.9 ± 2.2	20.4 ± 4.2	16.1 ± 3.6	17.1 ± 3.54	7.1 ± 2.9	9.4 ± 2.2
(mg g-1 FW ± SD)
Sugars (mg g-1 FW ± SD)
Rha	0.6 ± 0.05	0.4 ± 0.04	0.17 ± 0.01	0.13 ± 0.02	0.15 ± 0.02	0.12 ± 0.02	trace	trace
Fuc	0.2 ± 0.02	0.2 ± 0.01	trace	trace	trace	trace	trace	trace
Ara	5.1 ± 0.29	5.0 ± 0.3	1.5 ± 0.1	1.4 ± 0.2	1.2 ± 0.09	1.1 ± 0.05	0.3 ± 0.03	0.4 ± 0.02
Xyl	0.9 ± 0.1	1.1 ± 0.02	0.4 ± 0.07	0.4 ± 0.05	0.4 ± 0.04	0.5 ± 0.03	0.3 ± 0.02	0.3 ± 0.01
Man	0.5 ± 0.03	0.7 ± 0.05	0.2 ± 0.02	0.2 ± 0.02	0.1 ± 0.02	0.1 ± 0.01	trace	trace
Gal	5.5 ± 0.4	6.8 ± 0.5	2.3 ± 0.1	2.4 ± 0.4	1.1 ± 0.1	1.7 ± 0.06	0.3 ± 0.01	0.6 ± 0.08
Glc	0.7 ± 0.06	0.8 ± 0.1	0.3 ± 0.2	0.5 ± 0.02	0.3 ± 0.02	0.4 ± 0.13	0.14 ± 0.01	0.17 ± 0.02
UA	13.4 ± 2.1	7.0 ± 2.2	4.0 ± 0.3	3.6 ± 0.4	3.3 ± 0.8	3.1 ± 0.22	1.5 ± 0.1	2.2 ± 0.2
Total non-cellulosic sugars	26.8 ± 4.9	22.0 ± 2.4	9.0 ± 1.5	8.7 ± 1.7	6.6 ± 1.1	7.0 ± 0.48	2.6 ± 0.07	3.8 ± 0.08
C-Glc	16.4 ± 3.3	11.7 ± 2.5	4.5 ± 0.5	6.0 ± 0.1	4.6 ± 0.4	5.3 ± 0.5	1.9 ± 0.1	2.6 ± 0.2

Yields are from one season, with extractions being carried out in triplicate. Sugar composition of non-cellulosic neutral sugars was obtained by TFA hydrolysis. ‘Total non-cellulosic sugars’ are the sum of the neutral sugars plus uronic acid (UA). Cellulosic glucose (C-Glc) content of the cell wall material was obtained by sulphuric acid hydrolysis. Values are means from three cell wall extractions with duplicate assays each ± standard deviation. FW: Fresh weight; Rha: Rhamnose; Fuc: Fucose; Ara: Arabinose; Xyl: Xylose; Man: Mannose; Gal: Galactose; Glc: Glucose; UA: Uronic acid.