Figure 1.

p53 family members can transactivate p53-REs found in miR-associated promoter regions. A-B) Transactivation potential of p53 protein tested on a panel of 15 putative p53-dependent miR-REs and miR-34a-RE (a positive control) using the yeast functional assay. The expression of p53 was modulated by increasing concentrations of galactose in the culture medium (A, 0.008%: a moderate p53 expression; B, 0.128%: high p53 expression). C-D) The same panel of p53 miR-REs was tested using the other members of the p53 family p63 and p73 (gray and black bars respectively). The expression of p63β and p73β isoforms was induced using two different concentrations of galactose (C, 0.008%: moderate expression; D, 1%: maximal induction). Results are presented as fold of induction calculated over an empty expression vector. Bars plot the averages and standard deviations of at least three independent biological repeats. (E) Western Blot establishing galactose-dependent expression of p53, p63 or p73 in yeast. PGK1 was used as reference.