Figure 2.

p53-REs found in miR promoters can be used to classify p53 germline alleles associated with Li-Fraumeni Syndrome. Five p53 missense mutants representative of partial function (A138S, C141Y, R337C) or loss-of-function (A138P, R175H) germline p53 alleles were tested for transactivation from 5 p53 miR-REs using the yeast functional assay. Yeast transformants were cultured over-night in selective medium and the luciferase activity was measured using the miniaturized assay format [43]. Bars represent the averages and standard deviations of at least three independent biological repeats.