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. Author manuscript; available in PMC: 2015 May 7.
Published in final edited form as: Nat Commun. 2014 Nov 7;5:5223. doi: 10.1038/ncomms6223

Figure 5. Binding analysis of position 98β mutants of selected TCRs.

Figure 5

Binding titrations of the indicated mutants of A6-X15 (a), RD1-MART1 (b), RD1-MART1HIGH (c), and RD2-MART1 (d). Mutants were stained with 12.3 nM, 37.0 nM, 111 nM, 33 nM, and 1 μM Tax and MART/HLA-A2 PE-conjugated streptavidin tetramers. Normalized percent max MFI is plotted against peptide/HLA-A2 tetramer concentration. This experiment was also performed with pepMHC-Ig dimers with similar results (Supplementary Fig. 9).