Table 3.
Analysis of Pairwise Genetic Interactions for Microcephaly Reveal Specific Loci that Sensitize the Phenotype in Zebrafish Embryos
| Gene 1 | Gene 2 | Z Score |
|---|---|---|
| acadvl | – | −0.98 |
| cldn7a | – | −0.75 |
| ctdnep1b | – | −0.35 |
| dvl2 | – | −0.41 |
| eif5a | – | −0.62 |
| gabarap | – | 0.61 |
| acadvl | cldn7a | −0.85 |
| acadvl | ctdnep1b | −0.06 |
| acadvl | dvl2 | −1.91∗ |
| acadvl | eif5a | −0.07 |
| acadvl | gabarap | −3.5∗ |
| cldn7a | ctdnep1b | −1.6 |
| cldn7a | dvl2 | −2.02∗ |
| cldn7a | eif5a | −1.3 |
| cldn7a | gabarap | −3.5∗ |
| ctdnep1b | dvl2 | −5.0∗ |
| ctdnep1b | eif5a | −1.3 |
| ctdnep1b | gabarap | −4.2∗ |
| dvl2 | eif5a | −1.1 |
| dvl2 | gabarap | −1.1 |
| eif5a | gabarap | −0.8 |
Zebrafish embryos were injected with suboptimal doses (1 ng) of morpholino and 50–100 embryos were scored at 4 dpf for the distance between eyes. The Z scores represent the difference in distance between eyes with respect to the control population; asterisk (∗) indicates Z scores demonstrating interaction that lead to microcephaly.