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. 2014 Oct 24;7(5):590–604. doi: 10.1016/j.tranon.2014.08.001

Figure 2.

Figure 2

Effects of 17-AAG and NVP-AUY922 on colony formation. Pancreatic cancer cell lines IMIM-PC-1, PANC-1, and CFPAC-1 (A) or colorectal carcinoma cell lines HT-29, SW620, LoVo, and Caco-2 (B) were seeded over agar plates in the presence of DMSO (control), 0.5 μM 17-AAG, or 0.1 μM NVP-AUY922. Colorectal cancer primary cultures HCUVA (C and D) were seeded over agar plates in the presence of DMSO (control), 0.5 μM 17-AAG, 2 μM 17-AAG, 0.1 μM NVP-AUY922, or 1 μM NVP-AUY922. Colonies were stained and counted after 14 days. Each treatment was performed in triplicate and error bars are the SEM. Difference between means was statistically significant (*P < .05, Mann-Whitney test).