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. Author manuscript; available in PMC: 2014 Nov 10.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jul 24;438(2):283–288. doi: 10.1016/j.bbrc.2013.07.056

Figure 2. GABARAPL1 and GATE-16 expression during neutrophil differentiation of NB4 APL and CD34+ primary cells.

Figure 2

(A,B) mRNA levels of GABARAPL1 (upper panels) and GATE-16 (lower panels) were measured in control and ATRA-treated NB4 (A) and NB4-R2 APL (B) cell lines at day 4 and 6 using qPCR. Values were normalized to the housekeeping gene HMBS and are given as n-fold mRNA expression relative to the control of day 4 and 6, respectively. M.W.U, *p<0.01. **p<0.001, *** p<0.0001. (C) Primary CD34+ progenitor cells showed markedly increased GABARAPL1 and GATE-16 mRNA levels upon in vitro neutrophil differentiation using G-CSF over a 12 day period. Values were normalized to the housekeeping gene HMBS and given as n-fold mRNA expression relative to the levels at day 0 upon in vitro neutrophil differentiation of CD34+ using G-CSF.