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. Author manuscript; available in PMC: 2014 Nov 10.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jul 24;438(2):283–288. doi: 10.1016/j.bbrc.2013.07.056

Figure 4. Knocking down GATE-16 impairs ATRA-induced autophagy in NB4 cells.

Figure 4

(A) Endogenous LC3B protein levels were analyzed using confocal microscopy at day 4 of ATRA only treatment or in in combination with Bafilomycin A1 in SHC002 control transfected cells or GATE-16 knockdown cells. (B) LC3B puncta were quantified using ImageJ software. (C) Western blot analysis of LC3-II expression in SHC002 or shGATE-16_247 expressing NB4 cells after 4 days of ATRA administration. Total protein was extracted and 30ng of protein was loaded. Immunoblots were incubated with LC3 antibody. GAPDH represents the loading control. (D) Endogenous ATG5 protein levels were analyzed in ATRA-treated NB4 cells using confocal microscopy at day 4. (E) The proteolysis rate in NB4 cells upon ATRA treatment was measured in 4 independent experiments. Cells were either incubated with complete medium or medium supplemented with ATRA, in the presence or absence of BafilomycinA1.