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. Author manuscript; available in PMC: 2014 Nov 10.
Published in final edited form as: Pharm Res. 2013 Aug 16;30(9):2232–2247. doi: 10.1007/s11095-013-1140-2

Fig. 6.

Fig. 6

Effect of CR on transcription factor activity and relative expression patterns of prototypical target genes of transcription factors in livers of lean and obese mice. (a) CR changes the transcription factor binding activity in livers of lean and obese (OB) mice. Ahr, Creb, Fxr, Nrf2 and Pxr binding activity in nuclear fractions was determined using a Procarta TF binding assay. The data is represented as average mean fluorescence intensity (MFI)/10 µg protein ± SEM (n =7 for AL and n =10 for CR) fold change over the fed controls. Groups without a common letter are considered significantly different from each other (p ≤0.05). (b) CR differentially affects expression of prototypical target genes of transcription factors in lean and OB mouse livers. Heat maps were created for visual pattern analysis using R statistical software. Gene expression is represented as fold change of lean and OB CR mice over respective AL groups.