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. 2014 Apr 1;1(1):e000013. doi: 10.1136/lupus-2014-000013

Figure 1.

Figure 1

Intracellular position of podocyte proteins after exposure to healthy or lupus nephritis plasma. Human podocytes were incubated for 48 h with heat-inactivated plasma from six lupus nephritis (LN) patients and six healthy donors. Cells were then fixed and stained for α-actinin and CD2AP (CD2-associated protein) using immunofluorescence staining (see ‘Methods”). Cells were examined by confocal microscopy, by two independent assessors and the number of cells with membrane staining was noted. Representative images showing α-actinin staining after culture with LN (A) and healthy (B) plasma. The percentage of cells with membrane staining was compared between the two groups using a t test (C). Representative images showing CD2AP staining after culture with LN (D) and healthy (E) plasma. The percentage of cells with membrane staining was compared between the two groups using a t test (F). No significant differences in distribution of α-actinin or CD2AP were found.