Fig. 2.

Effect of tunicamycin on tissue factor (TF) activity and antigen in peripheral blood mononuclear cells (PBMC). PBMC (2 × 10⁶ cells mL⁻¹) in serum-rich RPMI 1640 medium were stimulated with 100 ng mL⁻¹ LPS for 6 h in the presence or absence of varying concentrations of tunicamycin. Where tunicamycin was added, cells were preincubated with it for 1 h. (A) Immunoblot analysis using TF polyclonal antibodies; (B) TF activity on intact cells as determined in factor X activation assay; (C) TF antigen levels determined in ELISA. Data are mean ± SEM (n = 4). ns, not statistically significant.