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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: J Surg Res. 2014 Mar 20;190(1):55–63. doi: 10.1016/j.jss.2014.03.036

Fig. 5. Oridonin inhibits TGF-β induced pSmad activity and ECM.

Fig. 5

LX-2 cells were preincubated with oridonin (7.5 μmol/L) for 2 hours and then treated with TGF-β (2 ng/mL) for 18 hours, whole cell lysates were analyzed by Western blot with antibodies for phosphorylated-Smad2/3 (A) and type I collagen (B), fibronectin (C). GAPDH was used as loading control. Densitometric analyses of bands were quantified and data expressed as fold change of control normalized to GAPDH. (D) LX-2 cells were pretreated with 7.5 mol/L of oridonin for 1 hour and then treated with TGF-β (2 ng/mL) for 1 hour. Nuclear proteins were extracted and analyzed by EMSA with 32P-labeled SBE probe described in experiment procedures.