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A proposed model for bulky (R)-diol production by the M145A and F128A BmEH variants. The M145A or F128A mutation expanded the predicted product release site of BmEH, which would facilitate the hydrolysis of bulky NGE. Tyrosines (Tyr-144 and -203) and aspartic acid (Asp-97) were served as Lewis acid and Lewis base, respectively.
