. 2014 Oct 20;111(44):15717–15722. doi: 10.1073/pnas.1404915111

Table 2.

Kinetic parameters of the wild-type and M145A BmEH catalyzed reactions of (R)- and (S)-NGE

Enzyme	(S)-NGE			(R)-NGE			E [=(k_cat/K_M)_R/(k_cat/K_M)_S]
Enzyme	k_cat, s⁻¹	K_M, mM	k_cat/K_M, s⁻¹⋅mM⁻¹	k_cat, s⁻¹	K_M, mM	k_cat/K_M, s⁻¹⋅mM⁻¹	E [=(k_cat/K_M)_R/(k_cat/K_M)_S]
Wild type	0.039 ± 0.005	1.40 ± 0.42	0.028 ± 0.009	0.75 ± 0.08	0.87 ± 0.32	0.86 ± 0.33	31
M145A	0.23 ± 0.02	1.62 ± 0.32	0.14 ± 0.03	29.1 ± 3.1	1.01 ± 0.36	28.8 ± 10.7	206

Reactions were performed in 0.1 M potassium phosphate buffer at pH 7.0 with 10% DMSO as cosolvent and 0.02% Tween 80, and the (R)- and (S)-NGE concentrations were varied in the range of 0.3–10 mM.