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Fam20B-dependent Xyl phosphorylation markedly stimulates GalT-II activity. (A) Gel electrophoresis analysis of recombinant MBP–GalT-II purified from insect cell conditioned medium. (B) The relative time-dependent addition of Gal onto Gal-Xyl-Ben and Gal-Xyl(P)-Ben by GalT-II in the presence of UDP-[6-3H]Gal. (C) Schematic model depicting the function of Fam20B in GAG biosynthesis.
